In our laboratory, we seek to characterize the inflammatory and angiogenic phenotype of fibroblast-like synoviocytes isolated from synovial tissue “normal” or “reaction” (N/R) and osteoarthritis “inflammatory” (I).
Immunohistochemical analysis revealed in the I area a greater number of CD45+ (at the synovial intima and nodular inflammatory infiltrates) and VEGF+ (at the intima).
The diameter of blood vessels identified by von Willebrand factor also appears greater in this area.
In parallel with these results, we observed a significant increased (p<0,001) of IL-6, IL-8 and PGE2 production by synoviocytes from
the I area compared to synoviocytes from N/R area.
Finally, in comparison with synoviocytes of N/R area, synoviocytes of I
area produced significantly more VEGF (p<0,001) but less than TSP-1 (p<0,001).